Journal: Cells

Article Title: D-Cysteine Activates Chaperone-Mediated Autophagy in Cerebellar Purkinje Cells via the Generation of Hydrogen Sulfide and Nrf2 Activation

doi: 10.3390/cells11071230

Figure Lengend Snippet: Effect of Na 2 S on the amounts of Nrf2 and CMA-related proteins in AD293 cells. ( A ) Immunoblot analyses of Nrf2 and β-actin in cell lysates from AD293 cells treated with Na 2 S (10 μM) for 4 and 24 h. ( B ) Quantitative analyses of Nrf2 amounts from the immunoblot results shown in A. ( C ) Immunoblot analyses of CMA-related proteins (LAMP2A, Hsc70) and β-actin in cell lysates from AD293 cells treated with Na 2 S (10 μM) for 24 h. ( D ) Quantitative analyses of LAMP2A and Hsc70 amounts from the immunoblot results shown in C. ( E ) Immunoblot analyses of a CMA/mA substrate (MEF2D) and β-actin in cell lysates from AD293 cells treated with Na 2 S (10 μM) for 24 h. ( F ) Quantitative analysis of MEF2D amount from the immunoblot results shown in F. Whole blot images are presented in . Amounts of β-actin were used as internal controls for quantification. Numbers in the columns represent the number of samples. * p < 0.05, *** p < 0.001 (unpaired t -test).

Article Snippet: Anti-myocyte enhancer factor 2D (MEF2D) mouse monoclonal antibody (#610774) was obtained from BD Biosciences (San Jose, CA, USA).

Techniques: Western Blot

Journal: Cells

Article Title: D-Cysteine Activates Chaperone-Mediated Autophagy in Cerebellar Purkinje Cells via the Generation of Hydrogen Sulfide and Nrf2 Activation

doi: 10.3390/cells11071230

Figure Lengend Snippet: Effect of long-term treatment with D-cysteine on the amounts of Nrf2- and CMA-related proteins in cerebellar lysates from ICR mice. ( A ) Immunoblot analyses of Nrf2, NQO1, LAMP2A, Hsc70, MEF2D, and β-actin in cerebellar lysates from ICR mice daily treated with saline (Sal) and D-cysteine (100 mg/kg/day) for 10 weeks. Whole blot images are presented in . ( B ) Quantitative analyses of the amounts of Nrf2, NQO1, LAMP2A, Hsc70, and MEF2D shown in A. Amounts of β-actin were used as internal controls for the quantification. * p < 0.05, ** p < 0.01 vs. saline-treated mice (unpaired t -test, n = 5 in both saline- and D-cysteine-treated mice).

Article Snippet: Anti-myocyte enhancer factor 2D (MEF2D) mouse monoclonal antibody (#610774) was obtained from BD Biosciences (San Jose, CA, USA).

Techniques: Western Blot